The Pima County Sheriff’s Department announced DNA test results in the Guthrie case, providing new forensic insights that investigators say could clarify key evidence as the long-running investigation continues.

The Pima County Sheriff’s Department announced Tuesday that DNA recovered from a glove found near the site where 84-year-old Nancy Guthrie disappeared did not produce a match in the Federal Bureau of Investigation’s national DNA database. Sheriff Chris Nanos said the unknown male DNA profile was entered into the FBI’s Combined DNA Index System, commonly known as CODIS, for comparison against profiles of convicted offenders and other qualifying samples. However, the search did not return any hits. The development represents a setback in what has become one of the most closely watched missing-person investigations in Arizona. While the absence of a match does not eliminate the possibility of identifying a suspect through other means, it narrows one potential avenue for immediate identification. Additional DNA evidence recovered from Guthrie’s residence remains under forensic analysis as investigators continue to piece together what happened during the early morning hours of February 1.

“The DNA that was submitted to CODIS was from the set of gloves found two miles away. It did not trigger a match in CODIS and did not match DNA found at the property,” Sheriff Nanos said during a public update. He added that the DNA discovered at the home is still undergoing testing and that further forensic work is required before definitive conclusions can be reached. The glove in question was discovered roughly two miles from Guthrie’s Catalina Foothills residence and is believed to resemble gloves worn by a masked individual captured on surveillance footage the night she vanished. Investigators have been meticulously collecting and examining physical evidence as part of what authorities describe as a multi-agency criminal investigation. Although no arrests have been made, officials emphasize that the case remains active and that they are pursuing every available lead.

Nancy Guthrie, the mother of “Today” show co-host Savannah Guthrie, was reported missing after being dropped off at her home by a family member. Law enforcement officials believe she may have been abducted, citing signs of forced entry and the presence of blood evidence inside the residence. Sheriff’s officials have previously ruled out Guthrie’s immediate family members as suspects, underscoring that the investigation is focused elsewhere. The involvement of the FBI has brought additional forensic resources and analytical support to the case. “We’re hopeful that we’re always getting closer,” Nanos said, reiterating that although the CODIS search did not produce a match, it remains one step in a broader investigative process. Authorities continue to review tips from the public while coordinating with federal partners to expand the scope of their inquiry.

A significant lead has emerged regarding clothing and gear worn by the masked suspect seen in doorbell camera footage. According to Nanos, detectives believe that the apparel and face covering visible in the video were likely purchased at Walmart. In particular, the suspect’s black Ozark Trail Hiker backpack — prominently displayed in the footage — is sold exclusively by the retail chain. Investigators are working to identify the exact brands of clothing and accessories worn by the suspect and determine whether the items were purchased online or at a physical store location. Detectives have reportedly spent days reviewing surveillance footage from local Walmart stores in Tucson and surrounding communities in an effort to trace the buyer. Walmart has provided records of recent purchases of the backpack model, including both in-store and online transactions, though a company spokesperson declined to comment publicly on the ongoing investigation.

The backpack and other gear have become focal points as the search enters its third week. Federal investigators have also concentrated on the suspect’s mask, which appears lighter in the doorbell footage but is believed to be black — an effect attributed to the infrared capabilities of the Nest security camera that captured the images. The FBI has released still photographs of the suspect, describing him as a male of average build standing approximately 5 feet 9 inches to 5 feet 10 inches tall. Over the weekend, federal officials confirmed that a black glove recovered near Guthrie’s home appears consistent with the gloves worn by the suspect in the surveillance video. While the DNA from that glove has not yet yielded a database match, forensic specialists continue to analyze fibers, trace materials, and other potential evidence that could link the item to a specific individual.

Despite the lack of a breakthrough from CODIS, authorities remain cautiously optimistic. Investigations of this complexity often require patience, coordination, and incremental progress. Sheriff Nanos reiterated that the absence of a match does not mean the DNA is unusable; it may simply indicate that the individual has not previously been entered into the national database. As forensic technologies evolve, additional testing methods could provide new insights. In the meantime, investigators are urging anyone with relevant information to come forward. The case has drawn national attention not only because of Guthrie’s family connections but also because of the disturbing circumstances surrounding her disappearance. Law enforcement agencies continue to follow leads, review surveillance footage, and analyze forensic evidence in hopes of locating Nancy Guthrie and identifying whoever may be responsible.

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